Figure 6

Signal pathways coupled to the ATP-induced nNOSNT-YFP translocation. Effect of kinase inhibitors on the translocation of nNOSNT-YFP induced by NMDA, forskolin, and ATP. After a 30-min incubation of NGF-differentiated PC12N cells with vehicle or with 100 μM NMDA, 100 μM ATP, and 10 μM forskolin (FK) in the presence of 0.1 μM calphostin C (Cal C), 1 μM KN-62, 1 μM PP2, 50 nM H-89, 25 μM LY294002 (LY), 25 μM PD98059 (PD), 20 μM SB203580 (SB) or 25 μM roscovitine (RV), the translocation of nNOSNT-YFP was observed by confocal microscopy and quantified as described in the legend for Figure 1B. Data are presented as the mean ± SEM (n = 4). *p < 0.05 compared with vehicle; ^# p < 0.05 compared with NMDA, ATP, and FK.