Figure 7From: Translocation of neuronal nitric oxide synthase to the plasma membrane by ATP is mediated by P2X and P2Y receptors mRNA expression of purinergic receptors in PC12N cells and spinal cord. PC12N cells were incubated without (-) or with (+) 50 ng/ml NGF for 5 days. Total RNA was extracted from the cells and the spinal cord and subjected to RT-PCR using the primers shown in Table 1. PCR products were analyzed by 2% agarose gel electrophoresis and stained with ethidium bromide. β-Actin and G3PDH were used for PC12N cells and for the spinal cord as the control and total RNA without RT was used as negative control.Back to article page