Figure 1

Adenovirus-mediated expression of GAD65 in the trigeminal ganglion. (A) Nissl stain showing the anatomical organization of the rat trigeminal ganglion with elongated clusters of sensory neurons (arrows) lying between bundles of myelinated axons. (B) At high magnification only the nuclei of SGCs (arrows) that surround the unipolar sensory neuron (N) are visible. (C) The attenuated cytoplasm of the SGCs is visible using the glial-specific marker, glutamine synthetase (arrows, green). Arrowheads point to SGC nuclei. (D) A low magnification image of the whole ganglion (outlined by arrowheads) showing the extent of GAD65 immunopositive elements (green) following injection of adGAD65. Images E1-F3 are taken 6 days after injection of AdGAD65 into the trigeminal ganglion. (E1-E3) GAD 65 expression (E1, green) is found in SGCs identified by GLAST immunostaining (E2, red) and in other elongated profiles (arrows). E3 Combined image. Asterisk indicates the same unstained cell body of a sensory neuron. (F) An example of a GAD65 positive neuron cell body. (G1-G3) The GAD65 immunopositive elongated process (G1, green) are not double labelled with the neuronal marker NF160 (G2 red) indicating that they are not axons. G3 combined image. Arrows and arrowheads indicate the same element is each figure. H, High magnification shows that the GAD65 immunopositive elongated elements (arrow) are often associated with nuclei (arrowhead). Double labelling for P75 (I1-I3) and Sch2e (J1-J3), a Schwann cell marker, show some GAD65 immunolabeled profiles are double labelled with these markers identifying the elongated profiles as non-myelinating (P75 positive) or myelinating (Sch2e positive) Schwann cells. Arrows and arrowheads indicate the same elements in each figure. Scale bars: A = 1 mm; B, C = 20 m; D = 1 mm; E, F = 40 μm; G, H = 10 μm; I = 20 μm; J = 5 μm.