Figure 4

Involvement of cGMP signaling in the effects of DETA/NO on PGE2 release. A: Cells were incubated for 6 h with or without 100 μM DETA/NO. The guanylate cyclase inhibitor NS2028 was included as indicated. Data are expressed as pg/ml of PGE2 and are means ± SEM of n = 6 for each experimental group. * p < 0.05, *** p < 0.001 vs control; °°° p < 0.001 vs DETA/NO alone. B: Cells were incubated with the indicated concentrations of the stable and cell-permeable analog of cGMP, 8-Br-cGMP. Data are expressed as pg/ml of PGE2, as mean ± SEM of n = 6 replicates for each experimental group. *** p < 0.001 vs. controls.