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Figure 6 | Molecular Pain

Figure 6

From: Proinflammatory-activated trigeminal satellite cells promote neuronal sensitization: relevance for migraine pathology

Figure 6

COX-2 protein expression induced by IL-1β in trigeminal satellite cells. (A) Whole-cell lysates were prepared from control satellite cells or cell treated with either IL-1β or DETA/NO for 4 h (2 replicates per each treatment), and equal amounts of protein were analyzed by western blot analysis for COX-2. The same blots were subsequently probed for β-actin. (B) Quantitation of densitometry wherein COX-2 values are reflected relative to those for β-actin. Data are expressed as mean ± SEM of n = 2 replicates for each group, each assayed in triplicates. *** p < 0.001 vs. controls. Satellite cells were incubated in presence of 10 ng/ml IL-1β together with the selective COX2 inhibitor, NS398 (C) or the selective COX1 inhibitor, SC560 (D) for 8 h. Data are expressed as pg/ml of PGE2, as mean ± SEM of n = 5 replicates for each experimental group. *** p < 0.001 vs. controls; °°° p < 0.001 vs IL-1β alone.

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