Figure 7

Persistent effects of pro-inflammatory stimuli on satellite cell activation. Satellite cells were incubated for different times in presence of 10 ng/ml IL-1β (A) or 100 μM DETA/NO (C), and the levels of PGE2 in the medium were assayed by RIA. Then the cultures were washed and further incubated in fresh medium for an another 24 h (see Methods for details). PGE2 that had accumulated during this 24 h incubation was assayed in the various treatment groups, i.e., different times of initial treatment in the presence of IL-1β (B) or DETA/NO (D). Data are expressed as pg/ml of PGE2, as mean ± SEM of n = 6 replicates for each experimental group. **, p < 0.01; *** p < 0.001 vs. controls