Figure 8

Conditioned media from activated satellite cells enhances CGRP release from trigeminal neurons. Satellite cells were exposed to IL-1β for 4 h (A) or DETA/NO for 6 h (B), then washed and incubated in fresh medium for another 24 h, as described in Fig. 7. Aliquots of this second cycle of conditioned medium (lacking the initial activating stimuli) were applied to trigeminal neurons at a 1:1 dilution, and CGRP was measured. After 30 min, capsaicin (100 nM) was applied to evoke secretion of CGRP in trigeminal neurons that had been pre-exposed to conditioned medium or not. Data are expressed as pg/well/10 min of CGRP, as mean ± SEM of n = 6 replicates for each experimental group. ***, p < 0.001 vs. controls; °°° p < 0.001 vs. capsaicin alone.