Figure 8
A and B. Comparison of the effects of pregabalin alone or in the presence of ondansetron on the evoked neuronal responses to mechanical punctate (A) and thermal stimulation (B)of the peripheral receptive field in MIA rats (n = 7). Pregablin alone significantly inhibits the natural evoked neuronal responses. This inhibitory effect of the drug is lost when spinal 5-HT3 receptors are blocked. Data are expressed as the mean percentage of pre-drug control values ± S.E.M. * p < 0.05 significant difference compared with pre-drug baseline controls. C. Quantification of α 2 δ-1 and 5-HT3A subunit mRNA levels in ipsilateral relative to contralateral DRGs following MIA or saline injection. (C). Mean data for Q-PCR of α2δ-1 in pooled ipsilateral L3/L4 DRGs (ipsi 3+4) or pooled ipsilateral L5/L6DRGs (ipsi 5+6) from either sham (n = 10 and n = 9 respectively) or MIA animals (n = 15 or n = 7 respectively) 14 days after injection. (D). Mean data for Q-PCR of 5-HT3A in pooled ipsilateral L3/L4 DRGs (ipsi 3+4) or pooled ipsilateral L5/L6DRGs (ipsi 5+6) from either sham (n = 7 and n = 7 respectively) or MIA animals (n = 11 or n = 7 respectively). Data are normalized to the respective contralateral side and expressed as the mean percentage of control values ± S.E.M. * p < 0.05 significant difference compared with sham controls.