Figure 6
Sensitivity of P2X3 current to PIP 2 depletion in HEK293 cells is reversed by R356Q mutation. A) Pooled data of wild-type and mutant P2X3 responses to 10 μM ATP expressed in HEK293 cells (N = 5–11). B) Representative surface biotinylation data of wild-type and mutant P2X3 receptor expression. C) Sample traces showing 35 μM wortmannin-induced rundown of wild-type P2X3 currents in HEK293 cells. D) Quantitative results. Rundown of wild-type and R356Q mutant P2X3 current in response to 35 μM wortmannin incubation (N = 5–6). (**, P < 0.01).