Figure 7
From: Increased chemokine signaling in a model of HIV1-associated peripheral neuropathy
Chemokines increased [Ca2+]i levels in acutely isolated rat DRG cells following the injury schemes. The figure shows examples of responses of cells acutely isolated from rat DRGs ipsilateral to the gp120/hCD4 and following gp120/hCD4 and ddC nerve injury at POD 21. Under normal conditions, cells rarely responded to any chemokine, but did respond to other stimuli such as high K or capsaicin (A). However, there was an increased responsiveness of the cells, the majority of which could be characterized as neurons, by POD21 in both the gp120/hCD4 only (B) and the combination of gp120/hCD4 with ddC injuries (C). For all experiments, MCP1 (M), IP-10 (I) and SDF1 (S) were applied at a concentration of 100 nM. Capsaicin (C) and high K (K) were applied at concentrations of 100 nM, 50 mM, respectively.