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Figure 6 | Molecular Pain

Figure 6

From: Recombinant adeno-associated virus serotype 6 (rAAV2/6)-mediated gene transfer to nociceptive neurons through different routes of delivery

Figure 6

Global transduction profile following intrathecal delivery of rAAV2/6. C57Bl/6 mice were intrathecally injected with 2.6 × 105 tu rAAV2/6 and tissues analyzed for the vector dissemination. (A) Macroscopic epifluorescence of the whole spinal cord and intact DRG demonstrate transduction across lumbar, thoracic and cervical vertebral levels. (B) DRG were scored for eGFP expression (0 = zero, 1 = faint, 2 = weak, 3 = moderate and 4 = intense eGFP expression) (n = 6). qPCR analysis of vector genome copy number per cell in (C) DRG and (D) brain and spinal cord. (E) Native eGFP expression in spinal cord section containing intact meninges. eGFP is present in the dorsal horn and dorsal root but not within the subarachnoid space or membranes of the pia mater and arachnoid. M, meninges, DH, dorsal horn, DR, dorsal root. Scale bar: 50 μm. (F) qPCR against the rAAV2/6 genome in peripheral tissues.

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Molecular Pain

ISSN: 1744-8069

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