Figure 2
From: Involvement of S-nitrosylation of actin in inhibition of neurotransmitter release by nitric oxide
Inhibition of PACAP-stimulated dopamine release from PC 12 cells by SNAP. A. Concentration dependency of PACAP for dopamine release from PC12 cells. PC12 cells (4 × 105 cells/well) cultured on 24-well dishes were stimulated with various concentrations of PACAP for 5 min. B. Time courses of dopamine release from PC12 cells by PACAP. PC12 cells were stimulated for the indicated times with 10 nM PACAP in the absence (closed circles) or presence (open circles) of 10 μM imipramine, an inhibitor of catecholamine reuptake. C. Inhibition of PACAP-stimulated dopamine release by SNAP. PC12 cells were stimulated with 10 nM PACAP without (open columns) or with (closed columns) 100 μM SNAP in the presence of 10 μM imipramine for the indicated times. Dopamine released into the medium and cellular dopamine were measured by HPLC as described in "Methods." Dopamine (DA) release (mean ± SD, n = 3) was expressed as a percentage of total dopamine (7.2 ± 0.8 ng/well) in PC12 cells. *P < 0.05; **P < 0.01 vs. without SNAP.