Figure 3
From: Involvement of S-nitrosylation of actin in inhibition of neurotransmitter release by nitric oxide
No mediation of the cGMP/cGK pathway in inhibition of dopamine release by SNAP. A. Effect of inhibitors of the NO/cGMP/cGK pathway on attenuation of dopamine release by SNAP. PC12 cells (4 × 105 cells/well) were stimulated for 5 min with 10 nM PACAP without (open columns) or with (closed columns) 100 μM SNAP in the presence of 300 nM ODQ, 1 μM KT5823 or 1 μM glibenclamide. B. Effect of cGMP and cAMP on dopamine release stimulated by PACAP. PC12 cells were stimulated for 5 min with 10 nM PACAP in the presence of 100 μM SNAP, 100 μM 8-Br-cGMP or 100 μM 8-Br-cAMP. Dopamine was measured by HPLC, and dopamine (DA) released into the medium (mean ± SD, n = 3) was expressed as a percentage of total dopamine in PC12 cells as described in the legend for Figure 2. *P < 0.05; **P < 0.01 vs. PACAP alone. C. Expression of cGKI in tissues and PC12 cells. Aliquots (100 μg/lane) of supernatants after centrifugation of homogenates of tissues and PC12 cells at 10,000 × g for 30 min were applied to 7.5% SDS-PAGE and immunoblotted with anti-cGKI antibody.