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Figure 5 | Molecular Pain

Figure 5

From: Involvement of S-nitrosylation of actin in inhibition of neurotransmitter release by nitric oxide

Figure 5

Inhibition of dopamine release by SNAP through F-actin breakdown. A. Representative fluorescence images of F-actin distribution in PC12 cells. After PC12 cells (3 × 104 cells/well) had been treated for 5 d with NGF (50 ng/ml), they were incubated for 5 min with various concentrations of SNAP or 10 μM cytochalasin B (CB). After fixation with 4% paraformaldehyde, the cells were labeled with Alexafluora 488-phalloidin (green) for F-actin and anti-actin monoclonal antibody/Alexafluora 546-anti-mouse IgG (red) for total actin. Bar = 5 m. B. Effect of SNAP and cytochalasin B on F-actin formation. Fluorescence intensity of more than 40 cells/slide was quantified by ImageJ as described in "Methods." Data are the mean ± SEM of 3 independent experiments. *P < 0.05, **P < 0.01 vs. 0 M SNAP. C. Effect of cytochalasin B on dopamine release stimulated by PACAP. PC12 cells were stimulated for 5 min with 10 nM PACAP without or with 10 M CB, and dopamine release was measured as described in the legend for Figure 2. **P < 0.01 vs. without CB.

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