Figure 2

Intrathecal NMDA receptor antagonists reverse tactile allodynia in the TG mice. Memantine, an NMDA receptor antagonist, was injected intrathecally between L5/6 lumbar regions into adult TG and WT mice, and behavioral sensitivities to mechanical stimulation before or after drug treatments were assessed blindly as paw withdrawal thresholds (PWT) to von Frey filament stimulation in the hindpaw. A. Dose-dependent effects of intrathecal memantine for PWT in the TG and WT mice measured 120 min post i.t. injection. Data shown represent the means ± SEM from n = 7 for each group. ^## p < 0.01, ^### p < 0.001 compared with WT mice, *** p < 0.001 compared with the pretreatment level using two-way ANOVA followed by Bonferroni post-tests. B. Time-dependent effects of intrathecal memantine (2.0 mg/kg) on allodynia reversal in TG mice compared with saline treatment and similar treatments in WT mice. Data shown represent the means ± SEM from n = 7 for each group. * p < 0.05, ** p < 0.01 compared with the pretreatment level, ^$ p < 0.05, ^$$$ p < 0.001 compared with saline treatment in TG mice using two-way ANOVA followed by Bonferroni post-tests.