Figure 2

Nocifensive behavior in trkB.T1 wildtype and knockout mice. A. Mice were baseline tested for mechanical threshold and then randomized within genotype (wildtype, knockout; n = 12 per genotype) to receive a saline (n = 6 per genotype) or d4T (n = 6 per genotype) tail vein injection. Following tail vein injection, mice were tested at 1, 7, 14, 21 and 28d for the presence of mechanical sensitivity. * indicates T1 WT (blue circles) and T1 KO (green triangles) significantly different from saline controls; p < 0.05 by Repeated Measures ANOVA with Tukey post-hoc testing. B. Mice were baseline tested for thermal threshold and then randomized within genotype (wildtype, knockout; n = 12 per genotype); to receive saline (n = 6 per genotype) or CFA (n = 6 per genotype) hind paw injection. Following hind paw injection, mice were testing for thermal sensitivity at 3 h and 1, 3, 7, 14, 21, 28 and 35d.* indicates T1 WT (blue circles) and T1 KO (green triangles) significantly different than saline controls; p < 0.05 by Repeated Measures ANOVA with Tukey post-hoc testing. C. The hind paws from mice in each group tested in B. were measured using calipers and the thickness recorded in mm. D. Latency to licking, number of licking bouts and duration of licking following capsaicin administration were recorded and the mean and s.e.m. presented for each genotype (n = 6). *indicates p < 0.05 by t-test.