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Figure 1 | Molecular Pain

Figure 1

From: Differential role of the menthol-binding residue Y745 in the antagonism of thermally gated TRPM8 channels

Figure 1

The TRPM8-Y745H mutant is insensitive to menthol, but retains cold sensitivity. A, Western blot where the lanes represent lysates of HEK293 cells transfected with TRPM8-wt and TRPM8-Y745H. B, Representative traces showing calcium imaging experiments of HEK293 cells expressing TRPM8-wt or TRPM8-Y745H. Note that only the cells expressing TRPM8-wt respond to menthol. C, Summary histogram of experiments seen in B. Intracellular calcium increases were compared using repeated-measures 2-way ANOVA in combination with Bonferroni's post test with respect to the effect of the mutation on each condition: *** p < 0.001; n = 14 (TRPM8-wt) and n = 19 (TRPM8-Y745H).

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