Figure 5
Variable inhibition of the Y745H mutant channel by capsazepine, clotrimazole, econazole and imidazole. A-D, Dose-inhibition curves of various antagonists at the cooling-activated TRPM8-wt and TRPM8-Y745H channels: A, capsazepine (n = 17-23/13-29 wt/mut); B, clotrimazole (n = 7-17/12-25); C, econazole (n = 14-27/9-27); and D, imidazole (n = 12-21/13-16). E, Comparison of the IC50 of block of TRPM8-wt vs. TRPM8-Y745H by the above antagonists: capsazepine (CPZ); clotrimazole (CLOT); econazole (ECO). In panels A-D, the red traces represent the fits to the Hill equation. Error bars were used as weights in fitting. At each concentration, the block was compared between TRPM8-wt and -Y745H using Student's unpaired t-test, and indicated where significant by: *** p < 0.001; ** p < 0.01; * p < 0.05.