Figure 3
GABA A R-mediated synaptic transmission in the SDH and DDH. A representative traces showing continuous recordings of GABAAR-mediated mIPSCs (holding potential - 70 mV) in the presence of TTX (1 μm), CNQX (10 μm), and strychnine (1 μm) from an SDH (red traces) and a DDH neuron (blue traces). Note similar mIPSC frequency in the neurons from the two regions. B individual mIPSCs from traces in A (aligned at rise onset) showing amplitude variability of GABAAR-mediated mIPSCs in both SDH (red traces) and DDH (blue traces). Inset shows averaged mIPSCs normalised to the same amplitude (same neurons as in A). Note the slower decay time of GABAAR-mediated mIPSCs in SDH neurons. C overlayed group data histograms comparing amplitude distributions for GABAAergic mIPSCs in SDH (red) and DDH (blue) neurons (n = 14 and 18 for SDH and DDH, respectively). The overlap of the two distributions indicates that GABAAR-mediated mIPSC amplitudes are similar in SDH and DDH neurons. Inset shows data presented as cumulative distribution plots. D plots comparing group data for GABAAR-mediated mIPSC decay time-constant and frequency in SDH and DDH neurons. GABAAR-mediated mIPSC decay time-constants were significantly slower in SDH neurons, however, mIPSC frequency was similar in both regions.