Effect of methanandamide on GABA
R-mediated synaptic transmission in SDH and DDH neurons. A Plot showing GABAAR-mediated mIPSC frequency (holding potential - 70 mV) in an SDH neuron during bath application of methAEA (5 μm, upper bar). mIPSC frequency declines significantly in the presence of methAEA. Middle traces are averaged mIPSCs (n = 15) in control conditions and after 10 minutes in methAEA. Black bars above x-axis indicate when averaged mIPSCs were obtained. Plot on right summarizes proportional changes to GABAAR-mediated mIPSC properties in methAEA. mIPSC frequency is reduced and rise time is slowed, however, mIPSC peak amplitude and decay time constant are not altered. B Effect of methAEA on GABAAR-mediated mIPSCs in DDH neurons (presented in same format as A). methAEA significantly reduced GABAAR-mediated mIPSC frequency, however, mIPSC peak amplitude, rise time, and decay time-constant were not altered in DDH neurons. Each data point on left panels in A and B represent averaged instantaneous mIPSC frequency, binned in 15 s intervals.