Gly- and GABA
-subunit expression in the SDH and DDH. A plots summarising qPCR analysis for GlyR subunits. Bars represent relative expression of GlyR subunits in the SDH and DDH. The α1 and β subunits were the most highly expressed in both SDH and DDH, whereas expression of the α4 subunit was negligible. The α2 and α3 subunits were expressed at lower levels in both regions. Overall, α1, β, and α2-4 subunits were expressed at significantly different levels in both regions. Note, scale on the y-axis has been broken and expanded to facilitate comparison of subunits showing lower expression levels. B plots summarising qPCR analysis for GABAAR subunits. Bars represent relative expression of GABAAR subunits in the SDH and DDH. Overall, GABAAR subunit expression was more variable than that observed for GlyR subunits. No significant differences in expression levels were identified, however, the expression profile for each subunit was similar in both SDH and DDH (ie, higher expression of α2, α3, β3, and γ2).