Parvalbumin and pNF-H fluorescent double immunolabelling of rat DRG tissue from control and oxaliplatin-treated animals. Representative DRG sections displaying fluorescent immunolabelling of pNF-H (FITC; green) (A, B) and parvalbumin (Cy3; red) alone (C, D) and overlaid to determine the extent of co-expression (yellow) (E, F) in L5 DRG of animals treated with the control vehicle (A, C, E) or oxaliplatin (B, D, F). Oxaliplatin reduced pNF-H cell body staining and its co-localisation with parvalbumin. The cell bodies of immunoreactive neurons appeared smaller after oxaliplatin. The number of nerve fibres staining for pNF-H and cell bodies staining for parvalbumin appeared unchanged after oxaliplatin. Magnification = 100×.