Enhanced NMDA receptor-mediated transmission after injury. (A) NMDA receptor-mediated mEPSCs recorded in PAG neurons at a holding potential of -30 mV. Representative traces show NMDA receptor-mediated mEPSCs in the saline, Hyp, CFA, and CFA + Hyp treated mouse. (B) Cumulative frequency (left) and amplitude (right) histogram of the mEPSCs from the cells in (A). Filled circles, from a saline control mouse; open circles, Hyp treated mouse; filled trangles, from a CFA-injected mouse; open trangles, from a CFA + Hyp treated mouse. (C) Summary of mEPSCs frequency (left) and amplitude (right) in neurons from the saline, Hyp, CFA, and CFA + Hyp treated mice. Values were significantly different from saline control * p < 0.05; from CFA-injected alone # p < 0.05.