Effects of intraplantar POMC-MIDGE-NLS on inflammatory pain. (A) Paw pressure thresholds (PPT) were measured at 4 days after induction of inflammation (white bars), then control buffer (100 μl) or POMC-MIDGE-NLS (25-100 μg/100μl) were injected into inflamed paws and PPT were re-evaluated 24 h later (black bars). *p < 0.05, compared with control buffer at 24 h after its injection (one-way ANOVA, Bonferroni t-test); #p < 0.001, compared with respective PPT of inflamed paws before control buffer or vector injections (depicting the development of inflammatory hyperalgesia) (paired t-test). (B) Time-course was evaluated at 4 days after induction of inflammation before (0 h) and at 2-96 h after control buffer or vector injections (i.e. up to 8 days following induction of inflammation). *p < 0.05, compared with control buffer (two-way repeated measures ANOVA, Bonferroni t-test).