Comparison of POMC-MIDGE-NLS effects on nociceptive thresholds and END content in immune cells in inflamed paws. The figure shows three separate sets of experiments to test reproducibility (A, B, C). Control MIDGE-NLS (50 μg) and POMC-MIDGE-NLS (25-100 μg) were injected into inflamed paws at 4 days after induction of inflammation. Effects of vectors on paw pressure thresholds (PPT; upper panels) and on END content in immune cells (lower panels) were measured in parallel 24 h after their injections (black bars). PPT were also measured before vector injections (white bars). *p < 0.05, compared with control MIDGE-NLS at 24 h after its injection (one-way ANOVA, Bonferroni t-test).