Figure 1From: Upregulation of casein kinase 1ε in dorsal root ganglia and spinal cord after mouse spinal nerve injury contributes to neuropathic pain SNL upregulates CK1ε expression in the spinal cord. (A) Immunoblot performed with a rabbit polyclonal anti-CK1ε antibody in the Ca v 2.2+/+ and Ca v 2.2-/- spinal cord. L5/L6 segments ipsilateral to the surgery were used. (B) Expression of CK1ε protein, which is normalized by GAPDH loading control. *p < 0.05, SNL Ca v 2.2+/+ compared with sham Ca v 2.2+/+ (Student's t test), # p < 0.05, SNL Ca v 2.2-/- compared with sham Ca v 2.2-/- (Student's t test); n = 7 in each group. (C-E) Immunofluorescent micrographs of CK1ε-IR in L5 spinal cord from sham- (C) and SNL-operated (D) C57BL/6J mice, and SNL-operated Ca v 2.2-/- mice (E). Scale bar, 100 μm.Back to article page