Figure 1

SNL upregulates CK1ε expression in the spinal cord. (A) Immunoblot performed with a rabbit polyclonal anti-CK1ε antibody in the Ca v 2.2+/+ and Ca v 2.2-/- spinal cord. L5/L6 segments ipsilateral to the surgery were used. (B) Expression of CK1ε protein, which is normalized by GAPDH loading control. *p < 0.05, SNL Ca v 2.2+/+ compared with sham Ca v 2.2+/+ (Student's t test), # p < 0.05, SNL Ca v 2.2-/- compared with sham Ca v 2.2-/- (Student's t test); n = 7 in each group. (C-E) Immunofluorescent micrographs of CK1ε-IR in L5 spinal cord from sham- (C) and SNL-operated (D) C57BL/6J mice, and SNL-operated Ca v 2.2-/- mice (E). Scale bar, 100 μm.