Effect of SNL injury on the number of CK1ε-positive neurons and CK1ε-IR. (A, B) Percentage of CK1ε-positive neurons in the superficial (A) and middle (B) layers of the dorsal horn. NeuN-positive cells were regarded as neurons. Each value represents the mean ± SEM of 3-4 determinations. *p < 0.05, ***p < 0.001 (one-way ANOVA followed by Tukey's post hoc test). (C-E) Confocal photomicrographs of CK1ε expression in the superficial dorsal horn neurons from sham- (C) and SNL- (D) operated C57BL/6J mice, and SNL-operated Ca
2.2-/- mice (E). Scale bar, 10 μm. (F) Averaged intensities of CK1ε-IR in the superficial dorsal horn. *p < 0.05, ***p < 0.001 (one-way ANOVA followed by Tukey's post hoc test); n = 3 in each group.