Figure 1

Involvement of RyR in the induction of LTP of fEPSPs in the spinal dorsal horn in vitro. A, fEPSPs were recorded in the presence of 10 μM bicuculline methiodide and 1 μM strychnine. fEPSPs recorded 10 min before HFS served as control. After HFS, fEPSPs significantly increased (n = 24). The inserted traces were the original recordings (without averaging). a-b corresponds to the time points as indicated. B, The LTP of fEPSPs stably lasted for 3 h (n = 8). C, 20 μM ryanodine applied 1 h before HFS blocked the induction of LTP (P < 0.001) (control n = 7; ryanodine n = 8). The inserted traces were the original recordings. D, 10 μM dantrolene perfused 1 h before HFS blocked the induction of LTP (P < 0.001) (control n = 7; dantrolene n = 7), and this effect was reversible (P < 0.001) (washout n = 3). E, 10 mM dantrolene has no significant effect on the amplitude of fEPSP. Control: the amplitude of fEPSP recorded 5 min before dantrolene application (10 μM); Dantrolene: 30 min after perfusion of dantrolene. Inserts show original traces recorded 5 min before and 30 min after dantrolene. F, 10 μM dantrolene perfused 30 min after HFS had no significant effect on the established LTP (P = 1) (control n = 5; dantrolene n = 6). Data were shown as mean ± s.e.m. in all figures; n: the number of slices.