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Figure 4 | Molecular Pain

Figure 4

From: Ryanodine receptors contribute to the induction of nociceptive input-evoked long-term potentiation in the rat spinal cord slice

Figure 4

No effect of IP3R antagonist on induction of spinal LTP in vitro. A, IP3R antagonist 2-APB (75 μM) applied for 1 h before HFS of LT had no significant effect either on the induction of LTP of fEPSPs (P = 0.303) (control, n = 7; 2-APB, 45 min from baseline, n = 8), or on the established LTP of fEPSPs (P = 0.173) (control, n = 4; 2-APB, 135 min from baseline, n = 4). B, As a positive control, 2-APB blocked the induction of hippocampal LTP. In the hippocampal slice, 75 μM 2-APB perfused for 1 h before conditioning stimulation (100 Hz for 1 sec, 3 times at 10-sec intervals, mimicking spinal HFS) blocked the induction of hippocampal LTP (control, 30 min after induction of hippocampal LTP in the presence of 10 μM bicuculline methiodide, n = 4; 2-APB, 30 min after induction of hippocampal LTP in the presence of 10 μM bicuculline methiodide and 75 μM 2-APB, n = 5). Error bars represent s.e.m. n: the number of slices. **P < 0.01.

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