Figure 4
From: Nitric oxide synthase modulates CFA-induced thermal hyperalgesia through cytokine regulation in mice
Effects of pretreatment with the NOS inhibitors 7-NINA, AG, L-NIO and L-NAME on cytokine gene expression after CFA. All treatments prevented the increase of TNF (A, *P < 0.05 and ***P < 0.001; inhibitor + CFA vs NS + CFA) and IL-1β (B, **P < 0.01 and ***P < 0.001; inhibitor + CFA vs NS + CFA) gene expression induced by CFA. In contrast to the effects of AG and L-NIO, which reduced the increase of IL-10 gene expression at 6, 16, 24 h and at 6 and 16 h (C, ***P < 0.001; AG + CFA or L-NIO + CFA vs NS + CFA), respectively, both 7-NINA and L-NAME at 6 and 16 h significantly enhanced the increase of IL-10 (C, # P < 0.05 and ### P < 0.001; 7-NINA + CFA or L-NAME + CFA vs NS + CFA), after CFA. At each time point n = 4.