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Figure 2 | Molecular Pain

Figure 2

From: Transient Receptor Potential Vanilloid 1 is essential for cisplatin-induced heat hyperalgesia in mice

Figure 2

Platinum drug induced TRPV1 and TRPA1 mRNA up-regulation in vitro and in vivo. In vitro and in vivo expression of TRV1, TRPM8 and TRPA1 mRNA using real-time RT-PCR are shown. Specific TaqMan primers and probe sets were used for the rat or mouse mRNA of TRPV1, TRPM8, TRPA1, and 28S RNA. The mRNA levels of TRP ion channels are illustrated after normalization to 28S RNA using the 2-ΔΔCT method [87] and presented as the ratio of fold change compared to vehicle-treated cells or tissues. In vitro fold increases of TRV1 (A) TRPM8 (B) and TRPA1 (C) mRNA from rat DRG neurons treated with cisplatin, oxaliplatin, or vehicle across time points 6, 12, 24 and 48 h are shown. Data represents means ± S.E.M from cDNA prepared from triplicate RNA samples. (D) In vivo fold increases of TRPV1, TRPM8 and TRPA1 mRNA from TG tissues from cisplatin and oxaliplatin-treated mice compared to vehicle-treated mice after a 3-week drug treatment are shown. Each sample was from pooled TG from three C57BL/6J wild-type mice after 3-week treatment with cisplatin, oxaliplatin or vehicle. Data represents means fold increases ± SEM from cDNA prepared from triplicate RNA samples, (***P < 0.001, **P < 0.01, *P < 0.05, two-way ANOVA, post-hoc analysis).

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