Table 1 Oligonucleotide Sequences of Primers Used for Standard RT-PCR
Rat TRPV1 | NM_031982 | Forward | GGTGTGCCTGCACCTAGC | 107 bp | |
|---|---|---|---|---|---|
| Â | Â | Reverse | CTCTTGGGGTGGGGACTC | Â | |
Rat TRPM8 | NM_134371.2 | Forward | GCCCAGTGATGTGGACAGTA | 64 bp | |
| Â | Â | Reverse | GGACTCATTTCCCGAGAAGG | Â | |
Rat TRPA1 | NM_207608.1 | Forward | ATTTGCGGCCTGAGTTTTT | 68 bp | |
| Â | Â | Reverse | TCCATCATTGTCCTCATCCA | Â | |
Rat 28S RNA | V01270 | Forward | GACAGGTTAGTTTTACCCTACTGATGA | 74 bp | |
| Â | Â | Reverse | CCTGCGGTTCCTCTCGTA | Â | |
Rat β-actin | NM_031144.2 | Forward | CCCGCGAGTACAACCTTCT | 72 bp | |
| Â | Â | Reverse | CGTCATCCATGGCGAACT | Â | |
Mouse TRPV1 | NM_001001445 | Forward | TTGGATTTTCCACAGCCGTAGT | 155 bp | |
| Â | Â | Reverse | GAACTTGAACAGCTCCAGACATGT | Â | |
Mouse TRPM8 | NM_134252 | Forward | CCAAATCACAATGAGGTCACAGC | 185 bp | |
| Â | Â | Reverse | CTTCAGGTGTAAAGTCCTCTGTACACTT | Â | |
Mouse TRPA1 | NM_177781 | Forward | ATTGTTCTCATGAACTTACTGATTGGCT | 186 bp | |
| Â | Â | Reverse | CCTGGGTCTATTTGGATACACGAT | Â | |
Mouse β-actin | NM_007393 | Forward | ACCCACACTGTGCCCATCTA | 342 bp | |
| Â | Â | Reverse | GCCACAGGATTCCATACCCA | Â | |