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Table 3 Quantitative and qualitative analysis of microglia density within the dorsal horn and thalamus with cannabinoid interventions

From: Cannabinoid-mediated modulation of neuropathic pain and microglial accumulation in a model of murine type I diabetic peripheral neuropathic pain

Mouse Cohort

Microglial Density (number/mm2)

 

Ventral Lumbar Spinal Cord

Thalamic Nuclei

Non-Diabetic

  

Intraperitoneal

  

Saline

147.3 ± 17.2 (-)

99.2 ± 9.9 (-)

Intranasal

  

Saline

136.1 ± 19.2 (-)

105.3 ± 9.3 (-)

Diabetic

  

Intraperitoneal

  

Early Intervention

  

Cannabidiol

228.6 ± 15.3* (+)

116.5 ± 9.4 (+/-)

SR144528

316.6 ± 17.8 (+)

127.5 ± 11.3 (+/-)

Nabilone

309.3 ± 18.2 (+)

125.6 ± 10.5 (+/-)

WIN55212-2

305.7 ± 20.5 (+)

124.4 ± 9.7 (+/-)

SR141716A

314.7 ± 16.9 (+)

130.0 ± 10.4 (+/-)

Late Intervention

  

Cannabidiol

314.2 ± 18.8 (+)

118.2 ± 10.8 (+/-)

SR144528

312.5 ± 19.4 (+)

125.6 ± 9.5 (+/-)

Nabilone

319.5 ± 19.9 (+)

124.5 ± 9.2 (+/-)

WIN55212-2

311.3 ± 18.9 (+)

131.2 ± 11.2 (+/-)

SR141716A

325.3 ± 17.7 (+)

129.0 ± 9.0 (+/-)

Intranasal

  

Early Intervention

  

Cannabidiol

209.5 ± 14.8* (+)

114.7 ± 12.2 (+/-)

SR144528

302.3 ± 18.2 (+)

125.4 ± 11.3 (+/-)

Nabilone

301.1 ± 16.5 (+)

132.5 ± 11.7 (+/-)

WIN55212-2

319.1 ± 18.4 (+)

126.1 ± 8.9 (+/-)

SR141716A

316.3 ± 17.8 (+)

130.5 ± 9.6 (+/-)

Late Intervention

  

Cannabidiol

314.5 ± 18.1 (+)

120.0 ± 9.5 (+/-)

SR144528

322.6 ± 20.5 (+)

133.4 ± 11.6 (+/-)

Nabilone

328.1 ± 19.9 (+)

125.6 ± 11.1 (+/-)

WIN55212-2

321.1 ± 18.0 (+)

122.0 ± 9.0 (+/-)

SR141716A

312.6 ± 18.4 (+)

124.6 ± 9.4 (+/-)

  1. All measures are mean +/- SEM for microglial densities. * indicates significance at p < 0.05 with comparison of diabetic mice cohort groups receiving high and low dose cannabidiol through either intranasal or intraperitoneal routes (non-matched ANOVA tests, F-values range between 1.89-3.92 for indicated groups and time points, DF ≥ 7,4, n ≥ 4 at each time point. Microglial activation is indicated using a qualitative scale: - for baseline staining; + for mild response; and ++ for moderate response, with the statistical mode response indicated in brackets, or where two values were similar, the greater response is shown in front of the second most common value.