Analysis of ultrasound vocalizations (USVs) and evoked mechanical hypersensitivity following spared nerve injury (SNI). C57Bl6 wild-type mice after SNI or sham treatment were tested at 50 kHz or 37 kHz recording frequencies. SNI- or sham-operated mice which had not been acclimatized to the setup did not show differences in USVs (A and B) (n = 9 mice/group). SNI- or sham-operated mice which had been pre-acclimatized to the recording chamber (C and D) showed differences in USV rate over basal state (pre-surgery values; †, p < 0.05) or with respect to each other (*, p < 0.05); ANOVA followed by post-hoc Fisher's test (n = 5 mice/group). Paw withdrawal responses to graded pressure applied via von Frey hairs prior to and following SNI (E) or sham treatment (F) are shown (n = 5 mice/group).