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Figure 6 | Molecular Pain

Figure 6

From: Down-regulation of Toll-like receptor 4 gene expression by short interfering RNA attenuates bone cancer pain in a rat model

Figure 6

Attenuation of TLR4 expression by Intrathecal TLR4 siRNA 439 . (A, B) The TLR4 mRNA levels in the L4~6 lumbar spinal cord from mismatch siRNA- and vehicle- treated bone cancer pain rats were higher in IBCP group (A) and in WBCP group (B) (n = 4, ANOVA1w, P < 0.01, post hoc Dunnett testing, df = 3, F = 1.12)relative to that of normal rats. TLR4 siRNA439 (SITLR4) treatment caused a significant decrease in IBCP group, and in WBCP group in the TLR4 mRNA level compared with that in normal rats (P < 0.01). (C, D, E, F) Data from several western blotting experiments revealed a significant up-regulation of TLR4 (n = 4, ANOVA1w, P < 0.01, post hoc Dunnett testing, df = 3, F = 1.16) protein in mismatch siRNA-and vehicle- treated bone cancer pain rats relative to that from normal rats, whether in the IBCP group (C, E) or in the WBCP group (D, F). TLR4 siRNA439 treatment caused a significant decrease (n = 4, ANOVA1w, P < 0.01, post hoc Dunnett testing, df = 3, F = 0.96)relative the level in mismatch siRNA-and vehicle- treated bone cancer pain rats, and a significant decline compared to normal rats (P < 0.05) in the IBCP group (C, E). Values are presented as mean ± SEM. *P < 0.05, **P < 0.01 vs. normal group; P < 0.05, ▲▲ P < 0.01 vs. vehicle-treated group; P < 0.05, P < 0.01 vs. mismatch siRNA-treated group.

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