mNT5E dephosphorylates AMP and can be inhibited by α,β-me-ADP. (A) Plot of initial velocity at the indicated concentrations of AMP at pH 7.0. Reactions (n = 3 per point) were stopped after 3 min. (B) The indicated concentrations of α,β-me-ADP were added to reactions (n = 3 per concentration) containing mNT5E (0.07 μg/μL) and 400 μM AMP at pH 7.0. (A, B) Inorganic phosphate was measured using malachite green. All data are presented as means ± s.e.m. Some error bars are obscured due to their small size. GraphPad Prism 5.0 was used to generate curves.