Figure 4From: Gi- and Gq-coupled ADP (P2Y) receptors act in opposition to modulate nociceptive signaling and inflammatory pain behavior The inhibitory effect of ADP is enhanced in the absence of P2Y1 signaling. The magnitudes of depolarization-evoked Ca++ transients in sensory neurons were measured before, and 5, 10 and 15 minutes after agonist application. Ca++ transients were not affected by application of buffer alone (dashed lines), but were significantly reduced after application of ADP (A-B), IDP (C), or UDPG (D; solid lines). Inhibition was prolonged by application of the selective P2Y1 antagonist MRS2179 (A) and in neurons from P2Y1-/- mice (B). There was no effect of MRS2179 in P2Y1-/- neurons (B). Values are mean ± SEM; n = 10 mice/timepoint each treatment. *p < 0.02 versus control for each treatment.Back to article page