Figure 8

Anterograde transport of PAP-II in afferent fibers. (A, B) Immunostaining shows the presence of PAP-II in nerve fibers in the rat sciatic nerve and dorsal root that are proximal (PROX, arrowheads) to the DRG 24 h after nerve ligation, but absence in the distal (DIST) portion. The number of PAP-II-immunoreactive afferent fibers is increased in the proximal portion of ligated sciatic nerve and dorsal root from the rat that had undergone inflammation, whereas there is no obvious accumulation of the protein in the distal side. Scale bar = 50 μm. (C) Statistical analysis shows that the accumulation of the PAP-II in the proximal side of dorsal root is increased by ~200%, and that in the sciatic nerve is increased by ~100% (the immunofluorescence intensity are measured in the dorsal root and sciatic nerve 500 μm from ligation site). * P < 0.05 versus contralateral side (n = 5 rats).