Figure 6From: NR2 subunits and NMDA receptors on lamina II inhibitory and excitatory interneurons of the mouse dorsal horn Effect of NR2A and NR2B antagonists on synaptic NMDA receptors. A, Representative traces show the inhibiting effects of EAB-318 (200 nM), ifenprodil (3 μM) and CPP (200 nM) on NMDA EPSCs evoked by focal stimulation and recorded from 3 EGFP+ neurons. Cells were held at +50 mV while EPSCs were recorded. B, shows the summary of NMDA receptor pharmacology recorded from EGFP+ and EGFP- neurons. The percentages of inhibition by EAB318, ifenprodil, and CPP on synaptic NMDA EPSCs were not significantly different between EGFP+ and EGFP- neurons (ns; not significant). We were able to obtain washout data on several of these neurons. The washout of antagonists ranged from 25% to 100% recovery of the blocked portion of the original NMDA EPSC amplitude. For the EGFP+ neurons, we were able to hold the neuron under study long enough to show washout in 6/16 neurons exposed to EAB318, 2/11 to CPP and 1/8 to ifenprodil. For the EGFP- neurons, we recorded partial to full recovery from 5/20 neurons exposed to EAB318, 2/14 neurons exposed to CPP and 2/8 neurons exposed to ifenprodil.Back to article page