Figure 8

SB203580 treatment inhibits protein expression of inflammatory mediators in db/db mice. A: Representative immunoblots of LDRG from db+ and db/db mice. After 1 wk treatments with vehicle control or vehicle with SB203580, immunoblots were performed for iNOS, COX2, and TNF-α. Increased protein levels for all three inflammatory mediators were demonstrated in LDRG of db/db mice compared to db+ littermates. This upregulation was inhibited by treatment with SB203580. Actin served as a loading control. B, C, D: Densitometric analysis of COX2 (B), iNOS (C), and TNF-α (D) demonstrated significant upregulation of each respective protein in db/db mice that is inhibited by SB203580. N = 4, *p < 0.05, **p < 0.01.