Figure 1
From: Effects of ranolazine on wild-type and mutant hNav1.7 channels and on DRG neuron excitability
Voltage-dependence of WT and the L858H and V1298F mutant channels. (A) Superimposed activation traces recorded from a representative HEK + hNav1.7r-WT expressing cell. (Average peak current is -4.2 ± 0.7 nA, n = 12) (B) Superimposed fast-inactivation traces recorded from the same cell as in (A). (C) Superimposed activation traces recorded from a representative HEK + hNav1.7r-L858H expressing cell. (Average peak current is -2.3 ± 0.3 nA, n = 12). (D) Superimposed fast-inactivation traces recorded from the same cell as in (C). (E) Superimposed activation traces recorded from a representative HEK + hNav1.7r-V1298F expressing cell (Average peak current is -1.9 ± 0.3 nA, n = 18). (F) Superimposed fast-inactivation traces from the same cell as in (E). Insets illustrate the voltage pulse protocols for activation and fast-inactivation. The bold bars indicate the portion of the data sweeps displayed in this figure. (G) Normalized conductance-voltage (G-V) curves are constructed from the averages of individual HEK 293 cells expressing WT (black squares, n = 12), the IEM mutation L858H (red circles, n = 12), or the PEPD mutation V1298F (blue triangles, n = 18). (H) Normalized fast-inactivation curves are constructed from the averages of the same cells as in panel G.