Figure 1

Spontaneous and movement evoked pain-related behaviors and mechanical hyperalgesia following sarcoma implantation in the femur. Time course of the number of spontaneous flinches of the cancer-bearing hind limb (A), unimpaired limb use score during spontaneous ambulation (B) and weight bearing score during standing (C) following sarcoma implantation (n = 6 for each test, closed circles). *Significantly different from the values obtained from sham-operated mice (n = 6 for each test, open squares), P < 0.05 (the Kruskal-Wallis test followed by the Scheffe test). Withdrawal thresholds to von Frey filament stimulation to the hind paw skin ipsilateral to cancer-bearing femur at 14 and 21 days after sarcoma implantation (n = 14) (D). *Significantly different from the values for the stimulation applied to the contralateral paw of cancer-bearing mice (n = 14) and those obtained from control mice (n = 20), P < 0.05 (non-repeated measures ANOVA by post hoc test or Dunnett's test). Values represent means ± S.E.M.