Figure 6

Analysis of the effects of BDNF on mEPSCs of delay neurons ( in vitro ). A. Superimposed recordings of 3 min of mEPSC activity in a control delay neuron; average of events presented as superimposed white trace. B. Similar superimposed recordings from a delay neuron in a BDNF-treated culture. C. Averaged events from the neurons illustrated in A and B. D. Averaged events normalized to control size. Note no change in the rate of decay of current. E. Distribution histogram (1 pA bins) for amplitudes of 1074 mEPSCs from control delay neurons. Fit of the data to three Gaussian distributions represented by black lines. F. Similar histogram and fit to three Gaussian functions for 1554 mEPSCs from BDNF treated neurons; arrow points out small number of very large events that appear in BDNF. Insets in E and F, graphs to show effect of number of Gaussian fits (peaks) on the value of χ 2 divided by the number of degrees of freedom. G. Superimposition of the three Gaussian peaks obtained in E with those obtained in F. Inset, comparison of area under curves for the three peaks. H. Data for control and BDNF mEPSCs > 30 pA replotted and compared on the same axes. I. Comparison of number of mEPSC events in control and BDNF treated larger than 30 pA. Modified from reference [24] and used with permission of the Physiological Society.