Expression of Runx1-dependent genes in Runx1-persistent versus Runx1-transient neurons in the adult lumbar DRG. (A) Double staining of Runx1 protein (green) and indicated RNA probe (red) on transverse sections of wild-type P30 lumbar (L5) DRG. Quantitative data are shown to the right of the panels. Note that 44.9% (231/515) and 9.2% (72/785) of Runx1+ neurons coexpressed Mrgprd and TRPM8, respectively. Note also that TRPV1high neurons represent 9% of total TRPV1+ neurons (33/356). Arrows indicate double-labeled neurons and arrowheads indicate single-labeled neurons. For TRPV1, asterisks indicate TRPV1low double-labeled neurons. (B) Schematic depicting the segregation of Runx1-dependent genes in the adult DRG into Program A and Program B, expressed predominantly in Runx1-persistent and Runx1-transient nociceptors, respectively.