Figure 4

Selective loss of Runx1-dependent genes in prospective Runx1-transient nociceptors in Tau-Runx1^F;Nav1.8^Cre mice. (A-C) In situ hybridization (ISH) using the indicated probes on sections through adult lumbar (L4/L5) DRG of Tau-Runx1^F control and Tau-Runx1^F ;Nav1.8^Cre mutant mice. For TrkA (C), both ISH (top pannels) and immunohistochemistry (IHC) (bottom panels) data are shown. Arrows indicate neurons expressing TRPV1^high and arrowheads indicate those with TRPV1^low. (D, E) Graph showing the average (± SEM) of the total number of neurons expressing the indicated probes per set of lumbar DRG sections of control (white bar) and mutant (gray bar) mice. The numbers of Mrgprd⁺, TRPM8⁺ and TRPV1^high neurons per set of sections (D) were not significantly changed in mutant versus control animals (249 ± 18 versus 243 ± 8 for Mrgprd⁺ neurons; 99 ± 6 versus 120 ± 9 for TRPM8⁺ neurons; and 25 ± 2 versus 29 ± 1 for TRPV1^high neurons) (p > 0.05). However, CGRP⁺ and TRPV1^low neurons in the mutants (E) decreased from 533 ± 19 to 120 ± 6 (***p < 0.001) and from 231 ± 18 to 82 ± 12 (***p < 0.001), respectively.