Figure 5
Effect of PKC activator PDBu on menthol responses. A) Sample images (left) and the trace to the right show fluorescence intensity in a cell at baseline, during menthol application, following treatment with 1 μM PDBu, and co-application of menthol with PDBu. B) Similar to A except BIM was applied together with PDBu and the test was on a different cell. C) Similar to B except staurosporine (Sta) was tested instead and the test was on a different cell. D, E) Pooled results show menthol responses before and after treatment with PDBu (n = 9), with PDBu in the presence of BIM (n = 13), with PDBu in the presence of Sta (n = 6), with BIM alone (n = 14), and with Sta alone (n = 24). Menthol responses were expressed as ΔF/F0 (D) and percent of menthol responses before treatment (E). In all cases 100 μM menthol was applied for 10 seconds. The PKC inhibitors BIM (1 μM) and Sta (0.5 μM) were pre-applied for 10 min and then co-applied with menthol. Maximum fluorescence intensity was used for menthol responses presented in D and E. Scale bars are 20 μm. Data are represented as mean ± SEM; *P < 0.05.