Short-term attenuation effects of pre-injected HDACI on thermal hyperalgesia in mice. Mice received intrathecally injected vehicle or HDACI at indicated dose 30 min before unilateral injection of CFA. Paw withdrawal latency was measured before injections as baseline, and after CFA injections as hyperalgesia response. Following HDACIs were injected for panel (in parenthesis) VPA (A), 4-BP (B), SAHA (C), TSA (D), LAQ824 (E). The inserted bar graphs showed dose dependence from the time lined up with a dash line. Mean values of eight animals per group plus standard errors are presented for the responses on the ipsilateral side. * P < 0.01, # P < 0.05: compared to vehicle at the same time point. G. Comparison of inhibition of thermal hyperalgesia by tested HDACI. Inhibition of hyperalgesia by all inhibitors at the maximal dose tested 30 min after CFA injection were calculated as described in method. One-way ANOVA analysis indicated there was no significant difference among HDAC-specific inhibitors, but all of them showed significant difference in comparison to VPA (P < 0.05).