Changes of HDACs' levels in the dorsal spinal cord after CFA injection into the hindpaw. The levels of HDACs in the dorsal spinal cord were analyzed by immunoblot using antibodies specific to each target as indicated. Examples of blots are shown in A and C. Digitally analyzed levels of each HDAC after normalization to β-actin are shown in B for class I HDACs and in D for class IIa HDACs. Mean values plus s.e. are averaged from 4 to 6 animals. Student t-test was used to examine the significance as indicated groups. * P < 0.01; ** P < 0.05.