Figure 4

Activation of GRP receptor by GRP reversibly increased sIPSCs in pyramidal neurons on the ACC. (A) The representative example of GRP (300 nM) modulation of sIPSCs in a ACC pyramidal neuron. The trace below represents sIPSCs recorded before, during and after GRP application. The bottom 3 traces are presented at an expanded scale. (B and C) Time course for the GRP-induced enhancement of sIPSC frequency and amplitude in the neuron shown in (A). Note the effect of GRP is reversible. (D) Statistical results showed the GRP-induced enhancement of sIPSC frequency and amplitude in the neuron shown in (A). Note the effect of GRP is reversible. *Indicates a significant difference between control and GRP. * < 0.05, ** < 0.01. (E) The facilitatory effect of GRP on sIPSC frequency is concentration dependent (30 nM, n = 6; 300 nM, n = 5; 1000 nM, n = 7). * < 0.05, ** < 0.01, *** < 0.001.