ATP7A protein expression in rat DRG tissue. (A) Detection of ATP7A protein by Western blot analysis in DRG of rats aged 4 weeks (lane 1), 12 weeks (lane 2) and 20 weeks (lane 3). Beta actin was probed as a loading control. (B) a and b, neuronal ATP7A immunoreactivity in rat L5 DRG tissue associated with cell bodies of small neurons, intense punctuate vesicular structures in cytoplasm (solid arrows), diffuse cytoplasmic and granular membrane staining (broken arrows), without staining of nuclei (n) or other tissue elements. b was the enlarged frame in a. Inset in a was a negative control. c, Fluorescent immunohistochemistry shows little overlap between ATP7A-immunoreactive (IR) neurons (green), phosphorylated neurofilament heavy subunit (pNF-H)-IR neurons (red), DAPI-stained satellite cells (asterisk) or nerve fibres (f). Scale bar, 20 μm.